Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene 259/1-7 [antikvár]

Immunology / Immunologie General Immunology, Applied Immunology/Allgemeine Immunologie, Angewandte Immunologie Lektorat: Prof. Dr. H. D. Brede Paul Ehrlich-Institut Frankfurt/Main VASQUEZ, Rosa and HERBERT, W. J. (Bact. Immunol., Univ., Glasgow Gil 6NT/U.K.). An Improved Method for Detection and Titration of Antibodies Cytophilic for Macrophages. - J. Immunol. Meth. 20, 77 - 85 (1978) The technique employs a series of small chambers formed on a microscope slide by the application of a plastic film in which holes have been punched. The procedure uses 5xl04 cells and 25pl of serum per chamber, and each test can be completed in 2 h. A permanent preparation results which can be examined as and when convenient. (4 fig., 2 tab., 7 ref.) (Be) ORR, W. and WARD, P. A. (Path., Univ. of Connecticut Hlth Cent., Parmington/ Conn. 06032). Quantitation of Leukotaxis in Agarose by Three Different Methods. - J. Immunol. Meth. 20, 95 - 107 (1978) Measurement of cells migrating in a single plane is preferable because this method is reliable and relatively simple. The assay conforms to a pattern determined by checkerboard analysis that confirms a true chemo-tactic response. H.D. Brede (Frankfurt/Main) PHATAK, A.G. (Sharada Res. Inst., Miraj/India). Various Methods of Lymphocyte Separation and their Relevance with the Formation of Non-Immune Rosettes. - J. Immunol. Meth. 20. 109 - 115 (1978) The percentage of non-immune rosettes varies according to the method of separation of lymphocytes. Buffy coat on Dextran-70 and Gravity Sedimentation method give the best rosette yields as compared with lymphocytes separated by centrifugation of heparinized blood (unsupported cen-trifugation) (P<0.001) or on Hypaque-Ficoll or Diaginol-Ficoll gradients (supported centrifugation) (P<0.05). The buffy coat separated on Dextran-70, layered on Ficoll-gradients (supported centrifugation) yields lower values of rosettes (P<0.05). (4 tab., 7 ref.) H.D. Brede (Frankfurt/Main) PARISH, C. R. and McKENZIE, I. F. C. (Microbiol., John Curtin School Med. Res., Australian Nat. Univ., Canberra, A. C. T./Australia). A Sensitive Resetting Method for Detecting Subpopulations of Lymphocytes which React with A11 oantis er a . - J. Immunol. Meth. 2 0, 173 - 183 (1978) The method entails reacting Ig-capped lymphoid cells with allo-antisera, and then assessing the uptake of alloantibodies by resetting the lymphocytes with SRBC coated with sheep IgG specific for mouse Ig. This resetting procedure was found to be generally more sensitive than the conventional dye exclusion microcytotoxicity test for detecting the binding of alloantibodies to lymphocytes. (2 tab., 5 fig., 15 ref.) H.D. Brede (Frankfurt/Main) CODING, J.W. (Walter and Eliza Hall Inst. Med. Res., Royal Melbourne Hosp., Parkville 3050/Australia). Use of Staphylococcal Protein A as an Immunological Reagent.-J. Immunol. Meth. 20, 241 - 253 (1978) Protein A is covalently linked to the cell wall of most strains of Staphylococcus aureus, and binds immunoglobulin molecules with high affi- 1 Zbl. Bakt. Hyg. , I. Abt. Ref. Bd. 259